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Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

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Question 1

Multiple Choice

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The specimen preparation that is best for viewing cell motility is ________.

A) hanging drop
B) fixed stained smear
C) Gram stain
D) negative stain
E) flagellar stain

F) C) and D)
G) C) and E)

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Question 2

Multiple Choice

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Culturing of the sputum resulted in the growth of distinct colonies and further isolation by subculturing is now needed. The RN understands that isolation is accomplished by taking a bit of growth from an individual colony and inoculating a separate medium, resulting in the production of a(n) ________.

A) simple culture
B) pure culture
C) isolated culture
D) mixed culture

E) A) and B)
F) B) and C)

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Question 3

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All of the following are examples of different types of microbiological media except ________.

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

If a microbiologist is studying a specimen at a total magnification of 950x, what is the magnifying power of the objective lens if the ocular lens is 10x?

A common medium used for growing fastidious bacteria is ________.

A reducing media is used to culture

The three physical forms of laboratory media are ________.

Cultures and specimens pose a potential health hazard and require proper handling and disposal via specific medical waste policies. Some facilities are regulated to maintain living catalogs of specimens that may be subcultured into a fresh medium for research and educational purposes. Such collections are referred to as ________.

Microscopic analysis revealed the presence of grapelike clusters of gram-positive cocci. The RN educates the patient that the species identification of the organism will require biochemical testing, which aids in microbial identification by providing information on cellular metabolism. Which of the following statements by the patient demonstrates understanding of the nurse's teaching?

The all-purpose media Tryptic Soy Agar (TSA) contains the following four ingredients; pancreatic digest of casein, digest of soybean meal, NaCl and agar. TSA is best described as ________.

Bacteria that require special growth factors and complex nutrients are termed ________.

A nutrient medium that has all of its chemical components identified, and their precise concentrations known and reproducible, would be termed ________.

Ms. Hungh's acid-fast stain results resulted as inconclusive for the presence of acid-fast bacilli. Culturing of the sputum is performed in order to isolate microbial growth for further analysis. Lowenstein-Jensen medium is utilized to select for the growth of Mycobacterium species if present in the sample, while suppressing unwanted background organisms. What is the proper term for this type of medium?

The patient asks how microbes from her body can be grown in the lab. The RN explains that specimens are introduced to nutrient medium and that any growth of the microbe that appears after incubating the specimen is called the ________.

Which type of microscope achieves the greatest resolution and highest magnification?

Agar is an important component of media because ________.

What type of isolation technique is most effective for the majority of applications?

The type of microscope in which you would see brightly illuminated specimens against a black background is ________.

The Gram staining procedure is best described as a(n) ________ staining technique.